Determination of Amylose Content by Iodine Affinity Titration The principle of iodine affinity titration: when the starch solution is potentiometrically titrated with iodine, there is no change in electrical properties (current, voltage) during the formation of complexes of iodine and starch, but potential (or current) is generated as soon as free iodine is present, and The change in potential (or current) can be seen, so that the amount of iodine that forms the complex can be determined from the potential (or current) titration curve and the amount of starch equivalent to the iodine binding amount can be calculated. The specific methods of determination are potentiometric titration and amperometric titration. Both of these methods require the use of pure amylose and amylopectin first, plotting a potential (or current) titration curve, and then titrating with a sample. The content of amylose and amylopectin in the sample. The affinity of iodine will vary with the source of the starch. Each starch has its own specific iodine affinity value. The value of the iodine affinity depends mainly on the amylose content in the starch. The higher the amylose content, the higher the iodine affinity value. The larger, the commonly used iodine affinity to determine the content of amylose in starch and as an indicator to assess the purity of amylose. Cao busy selected the potentiometric titration method to produce the standard working curve, and then directly found the percentage of amylose according to the volume of potassium iodate (KIO3) consumed at the end of titration of the starch sample. The results show that the method is simple, rapid, accurate and suitable for Determination of large batches of samples (eg breeding, census, etc.). Chen Junfang et al. compared iodine colorimetric method with potentiometric titration method, and considered that the standard working curve of potentiometric titration method was drawn by a number of function fitting methods. Compared with colorimetric method, it is more scientific and reasonable, accurate and reliable, and The correlation coefficient of the linear regression equation is closer to l, and the measured results are more stable and more reproducible. It is a scientific, reasonable, and effective method for the determination of amylose content. In addition, there are now more commonly used and more convenient direct determination using amylose content meter. Amylose content meter consists of amylose measuring instrument and computer detection system. The use of laser light source amylose content detector and CCD rice appearance quality image analysis and identification software system is an innovative achievement, it is the first in the country. The amylose analyzer can determine the amylose content of crops such as rice, corn, and wheat. In addition, the amylose meter passed the technical test of the Ministry of Agriculture's Grain Quality Supervision, Inspection and Testing Center and National Analytical Instrument Quality Supervision and Inspection Center. The results meet the standards of various quality inspection agencies in China. Ceramics Section Machine,Ceramic Cutting Machine,Ceramic Cutting Machinery,Cnc Finishing Machine Changzhou Kuaile Technology Co., Ltd , https://www.quartzglasssectionmachine.com